ABSTRACT
Grape virus diseases are a serious problem in Iran. Leaves and fruits of grape have been used for different purposes like cooking in Iran. The present investigation was carried out to study on the cytotoxic-activities of extracts of fruits and leaves of Vitis vinifera from both virus-free and virus-infected grape cultivars against breast cancer cell line [MDA-MB-231] and human embryonic kidney normal cell line [HEK 293]. In this experimental study, the considered grape cultivars were as follows: Rish Baba Sefid, Shahani Ghasre Shirin, Rotabi Zarghan, Asgari Najaf Abad, Fars, Kaj Angor Bojnord, Sarkesh Shiraz and Siahe Zarqan. A real-time multiplex polymerase chain reaction [real-time Multiplex PCR] assay was applied to detect virus infected cultivars. The cytotoxic effect of the methanol extracts of different Vitis vinifera varieties on cultured cells was monitored using [3- [4, 5-Dimethylthiazol-2-yl] -2, 5-diphenyltetrazolium bromide [MTT] assay at different concentrations [62.5, 125, 250, 500, 750, 1000 micro g mL[-1]]. Among these cultivars, Grapevine fanleaf virus [GFLV] along with related symptoms was detected in Siahe Zarqan and Fars. Methanolic extracts of leaves and fruits of Vitis vinifera from both virus free and virus infected cultivars showed a range of limited to moderate cytotoxic activity. However, methanol extract of leaves belonged to virus infected cultivars was found to have strong cytotoxic effect against MDA-MB-231 at different concentrations. Grapevine fanleaf virus [GFLV] can potentially increase the cytotoxicity of grape cultivars